Ph.D. position in characterization of dynamics of biological systems
Institut für Medizinische Physik und Biophysik, Medizinische Fakultät, Universität Leipzig
Part time (50%), start date as soon as possible
Understanding biological function depends critically on characterization of the dynamics of the system. However, direct measurement and characterization of a system’s motion is not trivial; in most cases, a single experimental method may not provide enough information to separate different types of motion present in a system, making interpretation challenging.
Our recent work has focused on developing analytical methods that allow comparison of timescale-specific dynamic parameters between different methods, with the result being the so-called detector analysis.1 We have applied detectors to solid- and solution-state nuclear magnetic resonance (NMR) and to molecular dynamics simulation (MD) data, giving us the ability to perform quantitative, timescale-specific comparison of simulation to experiment. MD simulation quality can be then validated against NMR experiments, and NMR may be interpreted based on MD.
NMR-based dynamics characterization primarily reports on the influence of motion on local bond orientations, where measured data is the collective result of both local and long-range motion. Förster Resonance Energy Transfer (FRET), on the other hand, reports on distance modulation between two fluorescent tags. Such data more directly characterizes long-range motion, therefore providing valuable experimental information that is not easily extracted from NMR alone.
Then, the successful candidate will work on further development of the detector analysis, with a particular focus on its application to FRET, with a goal of combining FRET results with both NMR and MD analyses. For further information regarding dynamics research using NMR and MD, visit: www.nmr4dynamics.de
-Masters in physics, chemistry, biochemistry, or similar
-Basic programming experience (or willingness to learn)
For further information, please contact Dr. Albert Smith
Telephone: +49 341 97-15773
Applications should include a cover letter, curriculum vitae, and university transcripts
1A.A. Smith, M. Ernst, B.H. Meier. “Because the light is Better Here: Correlation-Time Analysis by NMR Spectroscopy.” Angew. Chem. Int. Ed. 2017, 56, 13778.